Journal: Annals of Translational Medicine

Article Title: The effect of low frequency and low intensity ultrasound combined with microbubbles on the sonoporation efficiency of MDA-MB-231 cells

doi: 10.21037/atm.2020.02.155

Figure Lengend Snippet: Factors and levels of the experimental protocol

Article Snippet: Microbubble dose, irradiation time, and acoustic intensity were adjusted to observe differences between the bioeffects demonstrated. table ft1 table-wrap mode="anchored" t5 caption a7 Level Factors Sound intensity (a), mW/cm 2 Irradiation time (b), min Microbubble dose (c), mL 1 230 1 0 2 300 2 0.2 3 370 3 0.4 4 0.6 5 0.8 6 1 Open in a separate window caption a8 Factors and levels of the experimental protocol Fluorescence positive rate and cell survival rate After irradiation, the cells are washed with PBS 3 times, and the entry of fluorescent reagent FD4 at different treatment levels was observed under the EVOSTM M5000 Imaging System (Thermo Fisher Science, USA).

Techniques: Irradiation

Journal: Annals of Translational Medicine

Article Title: The effect of low frequency and low intensity ultrasound combined with microbubbles on the sonoporation efficiency of MDA-MB-231 cells

doi: 10.21037/atm.2020.02.155

Figure Lengend Snippet: FD4 fluorescent positive cells showed bright green fluorescence in the fluorescence mode, while non-stained cells did not. Fluorescence staining results in the blank control group, ultrasound irradiation groups and ultrasound irradiation combined with microbubble group are shown in A,B,C,D,E,F,G: (A) fluorescence staining result in the blank control group that microbubbles and ultrasound irradiation are not added; (B,C,D,E,F,G): irradiated by ultrasound (370 mW/cm2, 1 minute) and 0, 0.2, 0.4, 0.6, 0.8, and 1 mL microbubbles are added respectively.

Article Snippet: Microbubble dose, irradiation time, and acoustic intensity were adjusted to observe differences between the bioeffects demonstrated. table ft1 table-wrap mode="anchored" t5 caption a7 Level Factors Sound intensity (a), mW/cm 2 Irradiation time (b), min Microbubble dose (c), mL 1 230 1 0 2 300 2 0.2 3 370 3 0.4 4 0.6 5 0.8 6 1 Open in a separate window caption a8 Factors and levels of the experimental protocol Fluorescence positive rate and cell survival rate After irradiation, the cells are washed with PBS 3 times, and the entry of fluorescent reagent FD4 at different treatment levels was observed under the EVOSTM M5000 Imaging System (Thermo Fisher Science, USA).

Techniques: Fluorescence, Staining, Control, Irradiation

Journal: Annals of Translational Medicine

Article Title: The effect of low frequency and low intensity ultrasound combined with microbubbles on the sonoporation efficiency of MDA-MB-231 cells

doi: 10.21037/atm.2020.02.155

Figure Lengend Snippet: Positive rate of cell fluorescence staining at different levels of 3 factors: x-positive rate of fluorescence staining, (A) ultrasound intensity, (B) irradiation time, (C) microbubble dose.

Article Snippet: Microbubble dose, irradiation time, and acoustic intensity were adjusted to observe differences between the bioeffects demonstrated. table ft1 table-wrap mode="anchored" t5 caption a7 Level Factors Sound intensity (a), mW/cm 2 Irradiation time (b), min Microbubble dose (c), mL 1 230 1 0 2 300 2 0.2 3 370 3 0.4 4 0.6 5 0.8 6 1 Open in a separate window caption a8 Factors and levels of the experimental protocol Fluorescence positive rate and cell survival rate After irradiation, the cells are washed with PBS 3 times, and the entry of fluorescent reagent FD4 at different treatment levels was observed under the EVOSTM M5000 Imaging System (Thermo Fisher Science, USA).

Techniques: Fluorescence, Staining, Irradiation

Journal: Annals of Translational Medicine

Article Title: The effect of low frequency and low intensity ultrasound combined with microbubbles on the sonoporation efficiency of MDA-MB-231 cells

doi: 10.21037/atm.2020.02.155

Figure Lengend Snippet: Statistical analysis results (P values) of the 3 factors on the fluorescence staining positive rate respectively. a, ultrasound intensity; b, irradiation time; c, microbubble dose.

Article Snippet: Microbubble dose, irradiation time, and acoustic intensity were adjusted to observe differences between the bioeffects demonstrated. table ft1 table-wrap mode="anchored" t5 caption a7 Level Factors Sound intensity (a), mW/cm 2 Irradiation time (b), min Microbubble dose (c), mL 1 230 1 0 2 300 2 0.2 3 370 3 0.4 4 0.6 5 0.8 6 1 Open in a separate window caption a8 Factors and levels of the experimental protocol Fluorescence positive rate and cell survival rate After irradiation, the cells are washed with PBS 3 times, and the entry of fluorescent reagent FD4 at different treatment levels was observed under the EVOSTM M5000 Imaging System (Thermo Fisher Science, USA).

Techniques: Fluorescence, Staining, Irradiation

Journal: Annals of Translational Medicine

Article Title: The effect of low frequency and low intensity ultrasound combined with microbubbles on the sonoporation efficiency of MDA-MB-231 cells

doi: 10.21037/atm.2020.02.155

Figure Lengend Snippet: The optimal parameter combination of the 3 factors. (A) The positive rate of fluorescence at different sound intensities when the irradiation time was 2 minutes and 0.6 mL of microbubbles were added; (B) the positive rate of fluorescence at different irradiation times when the ultrasound intensity and microbubbles were 300 mW/cm2 and 0.6 mL respectively; (C) the positive rate of fluorescence at different microbubble concentrations when ultrasound intensity and irradiation time were 300 mW/cm2 and 2 minutes respectively.

Article Snippet: Microbubble dose, irradiation time, and acoustic intensity were adjusted to observe differences between the bioeffects demonstrated. table ft1 table-wrap mode="anchored" t5 caption a7 Level Factors Sound intensity (a), mW/cm 2 Irradiation time (b), min Microbubble dose (c), mL 1 230 1 0 2 300 2 0.2 3 370 3 0.4 4 0.6 5 0.8 6 1 Open in a separate window caption a8 Factors and levels of the experimental protocol Fluorescence positive rate and cell survival rate After irradiation, the cells are washed with PBS 3 times, and the entry of fluorescent reagent FD4 at different treatment levels was observed under the EVOSTM M5000 Imaging System (Thermo Fisher Science, USA).

Techniques: Fluorescence, Irradiation